Additional supplementary data for paper "A Three-Dimensional Model of the Yeast Genome"
Interaction files
Three-dimensional structure of the haploid yeast genome
- PDB file
(can be visualized with Rasmol)
- Rasmol script
to obtain Figure 5a (at the rasmol prompt, run "script rasmol_color_rotation1.script")
- Rasmol script
to obtain Figure 5b (at the rasmol prompt, run "script rasmol_color_rotation2.script")
- Text file with genomic positions and 3D coordinates.
To obtain the 3D coordinate of a particular locus, look for the closest
locus that appears in this file. This file can be used to obtain the
physical distance (in nm), according to our model, between specific
genomic loci. Note that the ribosomal DNA repeats in chromosome XII appear
only twice in the
reference genome, but in vivo this region is repeated 100-200
times. So we added this region 148 times to obtain 150 repeats. The
length of the region is 9100 bases, and the position is 451000. In
other words, our chromosome XII is 148*9100 bases longer than
the reference genome. All the genes that have locations greater
than 451000 in the reference genome should have 148*9100 bases added
to their coordinates to obtain the correspondence to our genes.
-
Code
for generating the 3D model
TRNA genes and clusters
Origins of replication (Di Rienzi et al., 2009)
Restriction enzyme sites, mappability and mid-fragments
- HindIII
- EcoRI
- Columns are: (1) chromosome; (2) Position of the RE site; (3) whether it is mappable (0=no, 1=yes); (4) the mid-fragment to the left; (5) the mid-fragment to the right
How to generate the Circos files
Note that the version of the yeast genome used for our analyses was
gi|144228165|ref|NC_001133.7.